PIERCE 15121-Reacti-Bind Streptavidin Coated Polystyrene Strip

产品名称：Reacti-Bind Streptavidin Coated Polystyrene Strip Plates, wi
产品货号：PIERCE 15121
产品规格：5 PLATES
产品价格：2086
产品说明书地址：http://www.piercenet.com/

销售直线：021-31322337、（何）

Introduction
The Thermo Scientific Pierce Streptavidin Coated Plates are made of polystyrene and are ideal for binding assays using biotinylated molecules. These plates are especially advantageous when direct adsorption to polystyrene plates denatures antibodies or the target molecule. Streptavidin has no carbohydrate groups and an isoelectric point of 5-6, resulting in low nonspecific interactions. The streptavidin coated plates are available in clear for colorimetric assays, white for chemiluminescent assays, and black for fluorescent assays.
Example ELISA Procedure
The following protocol describes a generalized enzyme-linked immunosorbent assay using a biotinylated capture antibody. Please see the reference list for other possible applications using streptavidin-coated microplates.
A. Materials Required
• Wash Buffer: Tris-buffered saline （25mM Tris, 150mM NaCl; pH 7.2; Product No. 28376）, 0.1% BSA, 0.05% Tween®-20 Detergent; alternatively, use Thermo Scientific Blocker BSA （Product No. 37520） supplemented with 0.05% Tween-20
• Biotinylated capture antibody adjusted to 10μg/mL, or other appropriate concentration, with Wash Buffer
• Antigen adjusted to appropriate concentration with Wash Buffer
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