PIERCE 15142/Nickel Coated, High Binding Capacity Plates

 产品名称：Pierce Nickel Coated, High Binding Capacity Plates （Clear, 8-well strip）
产品货号：PIERCE 15142
产品规格：5 PLATES
产品价格：2325
产品说明书地址：http://www.piercenet.com/
 

销售直线：021-31322337、（何）

Thermo Scientific Pierce Nickel Coated Plates provide a simple format to bind His-tagged fusion proteins for ELISA and other plate-based assays involving protein interactions of expressed recombinant proteins.

These Ni（2+） chelate-coated plates are ideal for analyzing polyhistidine-tagged fusion proteins by ELISA-based methods. Proteins that contain a succession of several histidine residues at the amino or carboxyl terminus have a strong binding affinity for metal. Bacterial lysates containing polyhistidine-tagged fusion proteins can be added directly to the plates without the need for blocking. The clear, white or black plates can be used with colorimetric, chemiluminescent or fluorescent detection methods, respectively.

Highlights:

• Ni²⁺ activated surface enables metal-chelate binding of His-tagged proteins
• Detergents used to lyse cells don't inhibit binding to activated plates as they do with plain polystyrene
• Better binding for sensitive assays compared to other commercially available nickel-activated plates
• Detection limit: 1ng of polyhistidine fusion protein
• Binding capacity: approx. 9pmol His-tagged protein （27kDa） per well
• Activation level: 200µL

Binding performance of nickel coated plates. Comparison of Thermo Scientific Pierce Nickel Coated Plates （Pierce） to Nickel-chelate plates from Brand Q in binding performance for His-tagged ATF fusion protein. Detection was accomplished with an HRP-conjugated anti-ATF antibody and TMB substrate.