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胶原蛋白酶 来源于溶组织梭菌

产品品牌:sigma Vetec 产品名称:胶原蛋白酶 来源于溶组织梭菌 别名: 梭菌肽酶 A 英文名称:Collagenase from Clostridium histolyticum Type I, for general use, Vetec™ reagent grade, powder, ≥125 CDU/mg solid 产品编号 V900891 | CAS号 9001-12-1
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产品介绍

胶原蛋白酶 来源于溶组织梭菌 胶原蛋白酶 来源于溶组织梭菌  

规格:100mg 1g

Biochem/physiol Actions
Collagenase is activated by four gram atom calcium per mole enzyme. It is inhibited by ethylene glycol-bis(beta-aminoethyl ether) - N, N, N′,N′-tetraacetic acid, beta-mercaptoethanol, glutathione, thioglycolic acid and 8-hydroxyquinoline.
Quality
也具有梭菌蛋白酶、非特异性中性蛋白酶和胰蛋白酶活性。
Unit Definition
One collagen digestion unit (CDU) liberates peptides from collagen from bovine achilles tendon equivalent in ninhydrin color to 1.0 μmole of leucine in 5 hours at pH 7.4 at 37 °C in the presence of calcium ions. One FALGPA hydrolysis unit hydrolyzes 1.0 μmole of furylacryloyl-Leu-Gly-Pro-Ala per min at 25°C. One Neutral Protease unit hydrolyzes casein to produce color equivalent to 1.0 μmole of tyrosine per 5 hr at pH 7.5 at 37°C. One Clostripain Unit hydrolyzes 1.0 μmole of BAEE per min at pH 7.6 at 25°C in the presence of DTT.
Application
Collagenase from Clostridium histolyticum may be used for the isolation of cells from many types of animal tissue. VETEC Collagenase Type I has the original balance of collagenase, caseinase, clostripain, and tryptic activities, and is suggested for epithelial, liver, lung and adrenal primary cell isolations.

性质

grade  Vetec reagent grade
form  powder
storage temp.  −20°C


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