总蛋白细胞裂解缓冲液，英文名称：Total Protein Cell Lysis Buffer，等级：蛋白组学级，包装规格：10ml，CAS：N/A

总蛋白细胞裂解缓冲液，英文名称：Total Protein Cell Lysis Buffer，等级：蛋白组学级，包装规格：10ml，CAS：N/A

M329
TOTAL PROTEIN CELL LYSIS BUFFER
Grade:PROTEOMICS GRADE
CAS Number: N/ASynonym(s): None
Molecular Weight: N/AMolecular Formula: N/A
Storage Condition: COLD
M329-10ML
Total Protein Cell Lysis Buffer is a nonionic detergent lysis buffer that enables efficient solubilization of the plasma and intracellular membranes, breaks weak intermolecular bonds, and solubilizes most proteins. Total Protein Cell Lysis Buffer reduces protein denaturation, protein complex disruption and loss of enzymatic activity since it contains less detergent than a typical RIPA lysis buffer. Simply, resuspend cells in Total Protein Cell Lysis Buffer and vortex vigoursly.  Incubate the cell suspension at 4 ºC with shaking for 30 minutes.  Vortex vigoursly for 30 seconds and pellet cellular debris.  The supernatant contains the total protein lysate.
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