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EZ-Link （tm） Sulfo-NHS-LC-LC-Biotin介绍

Thermo Scientific BCA蛋白定量产品可以用于研究蛋白：蛋白间相互作用，衡量亲和层析后的组分含量，估算从细胞裂解液里提取的膜蛋白含量和高通量筛选融合蛋白。

EZ-Link （tm） Sulfo-NHS-LC-LC-Biotin品牌介绍

美国Pierce公司（www.pierce.com）是蛋白质化学、免疫学和层析等很多方面的和*。http://www.thermo.com.cn/Category828.html

主要产品包括：

1）化学发光体和比色底物

2）分析试剂

3）荧光标记探针

4）酶联免疫分析试剂盒等

EZ-Link （tm） Sulfo-NHS-LC-LC-Biotin仅用于科研

Thermo Scientific EZ-Link Sulfo-NHS-LC-LC-Biotin is a long-chain biotinylation reagent for labeling antibodies, proteins and other molecules that have primary amines.

EZ-Link Sulfo-NHS-Biotin Reagents enable simple and efficient biotin labeling of antibodies, proteins and any other primary amine-containing macromolecules in solution. Specific labeling of cell surface proteins is another common application for these uniquely water-soluble and membrane impermeable reagents. Differing only in their spacer arm lengths, the three reagents offer the possibility of optimizing labeling and detection experiments where steric hindrance of biotin binding is an important factor.

Highlights:

• Label almost any antibody with biotin to facilitate immobilization, purification or detection
• Doubly long-chain （LC-LC） spacer arm （30.5 angstroms） reduces steric hindrance
• Amine reactive Sulfo-NHS ester reacts rapidly with any primary amine-containing molecule to attach the biotin label via a stable amide bond
• Sulfonate group attached to the biotinylation reagents increases reagent water solubility compared to ordinary NHS esters
• Sulfo-NHS-LC-LC-Biotin is water soluble, so biotin labeling can be done under physiologic conditions

   

   

• High purity, crystalline biotin labeling reagents can be used to create high-quality biotin-labeled derivatives