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EZ-Link (R) Biotin-PEG4-Hydrazide

货号:21360,名称:EZ-Link (R) Biotin-PEG4-Hydrazide,品牌:PIERCE,规格:50 MG
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产品介绍

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EZ-Link (R) Biotin-PEG4-Hydrazide介绍

 

Thermo Scientific BCA蛋白定量产品可以用于研究蛋白:蛋白间相互作用,衡量亲和层析后的组分含量,估算从细胞裂解液里提取的膜蛋白含量和高通量筛选融合蛋白。

 

EZ-Link (R) Biotin-PEG4-Hydrazide现货供应

 

PIERCE

21360

, 50 mg.

50 MG

PIERCE

21425

EZ-Link (R) Sulfo-NHS Biotinylation Kit

KIT

PIERCE

21435

EZ-Link (R) Sulfo-NHS-LC      Biotinylation Kit

KIT

 

品牌介绍

 

美国Pierce公司(www.pierce.com)是蛋白质化学、免疫学和层析等很多方面的先驱和*。http://www.thermo.com.cn/Category828.html

 

主要产品包括:

1)化学发光体和比色底物

 

2)分析试剂

 

3)荧光标记探针

 

4)酶联免疫分析试剂盒等

 

仅用于科研

 

Thermo Scientific EZ-Link Hydrazide-PEG4-Biotin (also called Biotin-PEG4-Hydrazide) is an aldehyde-reactive biotinylation reagent that contains a 4-unit polyethylene glycol (PEG) spacer arm for increased hydrophilicity.

Biotin-PEG4-Hydrazide enables biotinylation of glycoproteins and other molecules containing aldehydes or oxidizable sugar groups. The hydrazide group reacts spontaneously with aldehyde or ketone groups to form a stable hydrazone bond. Aldehyde groups can be created by gentle periodate oxidation of cis-diols in sialic acid and other sugars in carbohydrates. The hydrophilic, 4-unit polyethylene glycol (PEG) spacer arm imparts water solubility that is transferred to the biotinylated molecule, thus reducing aggregation of labeled proteins stored in solution. The PEG segment also adds length and flexibility to the spacer arm, minimizing steric hindrance involved with binding to avidin molecules.

Highlights:

  • Carbohydrate-reactive – forms stable hydrazone bond with periodate-oxidized sialic acid and other sugar groups in glycoproteins
  • Good choice for labeling polyclonal antibodies in the carbohydrate-rich Fc region, thereby avoiding steric hindrance of the antigen-binding sites
  • Use to biotinylate cell surface glycoproteins
  • PEGylation increases solubility and helps prevent aggregation of labeled molecules
  • PEG spacer arm reduces steric hindrance when binding to avidin molecules
  • Reactive group: hydrazide
  • Reactive toward: aldehyde and ketone groups (oxidized cis-diols in glycoproteins)



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