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DTSSP, 3,3'-Dithiobis (Sulfosuccinimidylpropionate)介绍
Thermo Scientific BCA蛋白定量产品可以用于研究蛋白:蛋白间相互作用,衡量亲和层析后的组分含量,估算从细胞裂解液里提取的膜蛋白含量和高通量筛选融合蛋白。
DTSSP, 3,3'-Dithiobis (Sulfosuccinimidylpropionate)现货供应
PIERCE | 21578 | DTSSP, 3,3'-Dithiobis (Sulfosuccinimidylpropionate) | 50 MG |
PIERCE | 21580 | BS3, Bis(Sulfosuccinimidyl) Suberate | 50 MG |
PIERCE | 21585 | BS3, No-Weigh(tm) Format, 8 x 2 mg microtubes | 8 X 2 MG |
DTSSP, 3,3'-Dithiobis (Sulfosuccinimidylpropionate)品牌介绍
美国Pierce公司(www.pierce.com)是蛋白质化学、免疫学和层析等很多方面的和*。http://www.thermo.com.cn/Category828.html
主要产品包括:
1)化学发光体和比色底物
2)分析试剂
3)荧光标记探针
4)酶联免疫分析试剂盒等
DTSSP, 3,3'-Dithiobis (Sulfosuccinimidylpropionate)仅用于科研
Thermo Scientific Pierce DTSSP is a popular water-soluble crosslinker that contains amine-reactive NHS-ester ends around an 8-atom spacer arm, whose central disulfide bond can be cleaved with reducing agents.
3,3´-Dithiobis(sulfosuccinimidylpropionate) (DTSSP) contains an amine-reactive N-hydroxysulfosuccinimide (sulfo-NHS) ester at each end of an 8-carbon spacer arm. Sulfo-NHS esters react with primary amines at pH 7-9 to form stable amide bonds, along with release of the N-hydroxysulfosuccinimide leaving group. Proteins, including antibodies, generally have several primary amines in the side chain of lysine (K) residues and the N-terminus of each polypeptide that are available as targets for sulfo-NHS-ester crosslinking reagents. DSS, the non-sulfonated analog of DTSSP is also available for applications that require a membrane-permeable crosslinker. BS3, the non-cleavable analog of DTSSP is also available for applications that require a stable spacer arm that cannot be cleaved in the presence of reducing agents.
DTSSP Highlights