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PIERCE,No-Weigh(tm) Format,8 x 2 mg microtubes

货号:21585,名称:BS3, No-Weigh(tm) Format,8 x 2 mg microtubes,品牌:PIERCE,规格:8 X 2 MG
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产品介绍

订货:

 

BS3, No-Weigh(tm) Format,8 x 2 mg microtubes介绍

 

Thermo Scientific BCA蛋白定量产品可以用于研究蛋白:蛋白间相互作用,衡量亲和层析后的组分含量,估算从细胞裂解液里提取的膜蛋白含量和高通量筛选融合蛋白。

 

BS3, No-Weigh(tm) Format,8 x 2 mg microtubes现货供应

 

PIERCE

21585

BS3, No-Weigh(tm) Format,8 x 2 mg microtubes

8 X 2 MG

PIERCE

21627

Streptavidin, R-Phycoerythrin Conjugated, 1 mL.

1 ML

PIERCE

21650

Sulfo-LC-SPDP, sulfosuccinimidyl 6-(3'-(2 pyridyldithio) pro

50 MG

 

BS3, No-Weigh(tm) Format,8 x 2 mg microtubes品牌介绍

 

美国Pierce公司(www.pierce.com)是蛋白质化学、免疫学和层析等很多方面的先驱和*。http://www.thermo.com.cn/Category828.html

 

主要产品包括:

1)化学发光体和比色底物

 

2)分析试剂

 

3)荧光标记探针

 

4)酶联免疫分析试剂盒等

 

BS3, No-Weigh(tm) Format,8 x 2 mg microtubes仅用于科研

 

Thermo Scientific Pierce BS3 (Sulfo-DSS) is bis(sulfosuccinimidyl)suberate, an amine-to-amine crosslinker that is homobifunctional, water-soluble, non-cleavable and membrane impermeable.

BS3 contains an amine-reactive N-hydroxysulfosuccinimide (NHS) ester at each end of an 8-carbon spacer arm. NHS esters react with primary amines at pH 7-9 to form stable amide bonds, along with release of the N-hydroxysulfosuccinimide leaving group. Proteins, including antibodies, generally have several primary amines in the side chain of lysine (K) residues and the N-terminus of each polypeptide that are available as targets for NHS-ester crosslinking reagents.

Because it contains the hydrophilic sulfonyl moiety, BS3 crosslinker is soluble up to ~10mM in water and many commonly used buffers, thus avoiding the use of organic solvents which may perturb protein structure. DSS, the non-water soluble analog of BS3 is also available for applications that require a less hydrophilic crosslinker (e.g., to effect intracellular crosslinking). DSS and BS3 have essentially identical crosslinking activity toward primary amines.

 

BS3 Highlights

  • Amine-reactive Sulfo-NHS ester reacts rapidly with any primary amine-containing molecule
  • BS3 is water-soluble, so crosslinking can be done in physiologic solutions
  • Water-soluble crosslinking reagent is ideal for cell-surface crosslinking
  • High-purity, crystalline BS3 can be used to create high-purity crosslinked conjugates

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