你的位置:首页 >产品展示 > 生化试剂 > 琼脂糖 > 21857PIERCE,N-Succinimidyl 3-(2-Pyridyldithio)Propionat

PIERCE,N-Succinimidyl 3-(2-Pyridyldithio)Propionat

货号:21857,名称:N-Succinimidyl 3-(2-Pyridyldithio)Propionate (SPDP),品牌:PIERCE,规格:50 MG
  • 在线留言
产品介绍

订货:

:  787994648

N-Succinimidyl 3-(2-Pyridyldithio)Propionate (SPDP)介绍


Thermo Scientific BCA蛋白定量产品可以用于研究蛋白:蛋白间相互作用,衡量亲和层析后的组分含量,估算从细胞裂解液里提取的膜蛋白含量和高通量筛选融合蛋白。


N-Succinimidyl 3-(2-Pyridyldithio)Propionate (SPDP)现货供应



PIERCE

21857

N-Succinimidyl 3-(2-Pyridyldithio)Propionate (SPDP)

50 MG

PIERCE

22122

Sulfo-SMCC, 1 g.

1 G

PIERCE

22305

MPBH: 4-(4-N-Maleimidophenyl)Butyric Acid Hydrazide Hydrochl

50 MG.


N-Succinimidyl 3-(2-Pyridyldithio)Propionate (SPDP)品牌介绍


美国Pierce公司(www.pierce.com)是蛋白质化学、免疫学和层析等很多方面的和*。http://www.thermo.com.cn/Category828.html

 

主要产品包括:


1)化学发光体和比色底物

 

2)分析试剂

 

3)荧光标记探针

 

4)酶联免疫分析试剂盒等


N-Succinimidyl 3-(2-Pyridyldithio)Propionate (SPDP)

仅用于科研


Thermo Scientific Pierce SPDP is a short-chain crosslinker for amine-to-sulfhydryl conjugation via NHS-ester and pyridyldithiol reactive groups that form cleavable (reducible) disulfide bonds with cysteine sulfhydryls.


Thermo Scientific Pierce LC-SPDP is a long-chain crosslinker for amine-to-sulfhydryl conjugation via NHS-ester and pyridyldithiol reactive groups that form cleavable (reducible) disulfide bonds with cysteine sulfhydryls.

We offer three analogs of SPDP: the standard version (SPDP), a derivative with a longer spacer arm (LC-SPDP) and Sulfo-LC-SPDP, the sulfonated analog of LC-SPDP is available for applications that require a water-soluble, membrane-impermeable crosslinker. SPDP (N -Succinimidyl 3-(2-pyridyldithio)-propionate) and LC-SPDP (Succinimidyl 6-(3-[2-pyridyldithio]-propionamido)hexanoate) are heterobifunctional, thiol-cleavable and membrane permeable crosslinkers. They each contain an amine-reactive N-hydroxysuccinimide (NHS) ester that will react with lysine residues to form a stable amide bond. The other end of the spacer arm is terminated in the pyridyl disulfide group that will react with sulfhydryls to form a reversible disulfide bond.

Highlights:

  • Releases a detectable by-product when reacted with a free sulfhydryl group; by measuring the release of pyridine-2-thione at 343 nm, the reaction can be easily followed
  • Amine reactive Sulfo-NHS ester reacts rapidly with any primary amine-containing molecule
  • Disulfide bond in the spacer arm is readily cleaved by 10-50 mM DTT or TCEP at pH 8.5
  • Spacer arm is also cleaved with 5% β-mercaptoethanol in SDS-PAGE sample loading buffer at 100°C for 5 minutes
  • Cleavable crosslinker allows separation of crosslinked products
  • SPDP crosslinker is membrane permeable, so crosslinking can be carried out inside the cell


  • 佰易聚生物商城提供优质的分子生物学试剂和试剂盒、美国联合碳化和美国光谱医学透析袋、培养基和培养耗材、细胞因子和细胞分离液、抗体和elisa试剂盒及常用生物试剂等,大量现货火爆*,欢迎登录www.ebioeasy.com


更多产品
在线留言

留言框

  • 产品:

  • 您的单位:

  • 您的姓名:

  • 联系电话:

  • 常用邮箱:

  • 省份:

  • 详细地址:

  • 补充说明:

  • 验证码:

    请输入计算结果(填写阿拉伯数字),如:三加四=7