PIERCE，（SULFO-SMCC） SULFOSUCCINIMIDYL4-（N-MALEIMID
- 型号： 22322
- 更新时间： 2024-03-22
- 上一个：22311PIERCE，（MBS） M-MALEIMIDOBENZOYL N-HYDROXYSUCCINIM
- 下一个：22324PIERCE，Sulfo-GMBS, N-gamma-maleimidobutyryloxysulf
订货：，，
787994648
（SULFO-SMCC） SULFOSUCCINIMIDYL4-（N-MALEIMIDOMETHYL）CYCLOHEXA介绍
Thermo Scientific BCA蛋白定量产品可以用于研究蛋白：蛋白间相互作用，衡量亲和层析后的组分含量，估算从细胞裂解液里提取的膜蛋白含量和高通量筛选融合蛋白。
（SULFO-SMCC） SULFOSUCCINIMIDYL4-（N-MALEIMIDOMETHYL）CYCLOHEXA
PIERCE | 22322 | （SULFO-SMCC） SULFOSUCCINIMIDYL4-（N-MALEIMIDOMETHYL）CYCLOHEXA | 50 MG |
PIERCE | 22324 | Sulfo-GMBS, N-gamma-maleimidobutyryloxysulfosuccinimide Este | 50 MG |
PIERCE | 22330 | 1,6-BISMALEIMIDOHEXANE, BMH | 50 MG |
（SULFO-SMCC） SULFOSUCCINIMIDYL4-（N-MALEIMIDOMETHYL）CYCLOHEXA品牌介绍
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（SULFO-SMCC）SULFOSUCCINIMIDYL4-（N-MALEIMIDOMETHYL）CYCLOHEXA用于科研
Thermo Scientific Pierce Sulfo-SMCC is a very popular, water-soluble, amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a medium-length cyclohexane spacer arm （8.3 angstroms）.
Sulfosuccinimidyl-4-（N-maleimidomethyl）cyclohexane-1-carboxylate （Sulfo-SMCC） is a water-soluble, non-cleavable and membrane impermeable crosslinker. It contains an amine-reactive N-hydroxysuccinimide （NHS ester） and a sulfhydryl-reactive maleimide group. NHS esters react with primary amines at pH 7-9 to form stable amide bonds. Maleimides react with sulfhydryl groups at pH 6.5-7.5 to form stable thioether bonds. The maleimide groups of Sulfo-SMCC and SMCC are unusually stable up to pH 7.5 because of the cyclohexane bridge in the spacer arm. Because it contains the hydrophilic sulfonyl moiety, Sulfo-SMCC is soluble up to ~10 mM in water and many commonly used buffers, thus avoiding the use of organic solvents which may perturb protein structure.
Sulfo-SMCC Highlights
- Amine reactive Sulfo-NHS ester crosslinks rapidly with primary amine-containing molecule
- Sulfhydryl-reactive maleimide reacts with cysteine residues to yield specific conjugates
- Sulfo-SMCC is water soluble, so crosslinking can be done in physiologic solutions
- High purity, crystalline Sulfo-SMCC can be used to create high-purity maleimide-activated derivatives
- Cyclohexane bridge confers added stability to the maleimide group making Sulfo-SMCC the ideal crosslinking agent for maleimide activation of proteins. Maleimide groups are stable for 64 hours in 0.1 M sodium phosphate buffer, pH 7 at 4°C.
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