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(SULFO-SMCC) SULFOSUCCINIMIDYL4-(N-MALEIMIDOMETHYL)CYCLOHEXA介绍
Thermo Scientific BCA蛋白定量产品可以用于研究蛋白:蛋白间相互作用,衡量亲和层析后的组分含量,估算从细胞裂解液里提取的膜蛋白含量和高通量筛选融合蛋白。
(SULFO-SMCC) SULFOSUCCINIMIDYL4-(N-MALEIMIDOMETHYL)CYCLOHEXA
PIERCE | 22322 | (SULFO-SMCC) SULFOSUCCINIMIDYL4-(N-MALEIMIDOMETHYL)CYCLOHEXA | 50 MG |
PIERCE | 22324 | Sulfo-GMBS, N-gamma-maleimidobutyryloxysulfosuccinimide Este | 50 MG |
PIERCE | 22330 | 1,6-BISMALEIMIDOHEXANE, BMH | 50 MG |
(SULFO-SMCC) SULFOSUCCINIMIDYL4-(N-MALEIMIDOMETHYL)CYCLOHEXA品牌介绍
美国Pierce公司(www.pierce.com)是蛋白质化学、免疫学和层析等很多方面的和*。http://www.thermo.com.cn/Category828.html
主要产品包括:
1)化学发光体和比色底物
2)分析试剂
3)荧光标记探针
4)酶联免疫分析试剂盒等
(SULFO-SMCC)SULFOSUCCINIMIDYL4-(N-MALEIMIDOMETHYL)CYCLOHEXA用于科研
Thermo Scientific Pierce Sulfo-SMCC is a very popular, water-soluble, amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a medium-length cyclohexane spacer arm (8.3 angstroms).
Sulfosuccinimidyl-4-(N-maleimidomethyl)cyclohexane-1-carboxylate (Sulfo-SMCC) is a water-soluble, non-cleavable and membrane impermeable crosslinker. It contains an amine-reactive N-hydroxysuccinimide (NHS ester) and a sulfhydryl-reactive maleimide group. NHS esters react with primary amines at pH 7-9 to form stable amide bonds. Maleimides react with sulfhydryl groups at pH 6.5-7.5 to form stable thioether bonds. The maleimide groups of Sulfo-SMCC and SMCC are unusually stable up to pH 7.5 because of the cyclohexane bridge in the spacer arm. Because it contains the hydrophilic sulfonyl moiety, Sulfo-SMCC is soluble up to ~10 mM in water and many commonly used buffers, thus avoiding the use of organic solvents which may perturb protein structure.
Sulfo-SMCC Highlights