PIERCE,DSP (Lomant's Reagent)
- 型号: 22586
- 更新时间: 2024-03-22
- 上一个:22585PIERCE,DITHIOBIS (SUCCINIMIDYL PROPIONATE),(DSP)
- 下一个:22589PIERCE,(SULFO-SANPAH) SULFOSUCCINIMIDYL 6-(4'-AZID
订货:,,
DSP (Lomant's Reagent)介绍
Thermo Scientific BCA蛋白定量产品可以用于研究蛋白:蛋白间相互作用,衡量亲和层析后的组分含量,估算从细胞裂解液里提取的膜蛋白含量和高通量筛选融合蛋白。
DSP (Lomant's Reagent)现货供应
PIERCE | 22586 | DSP (Lomant's Reagent) , 50 mg | 50 MG |
PIERCE | 22589 | (SULFO-SANPAH) SULFOSUCCINIMIDYL 6-(4'-AZIDO-2'-) NITROPHENY | 50 MG |
PIERCE | 22622 | Sulfo-SMCC, No-Weigh(tm) Format, 8 x 2 mg microtubes | 8 X 2 MG |
DSP (Lomant's Reagent)品牌介绍
美国Pierce公司(www.pierce.com)是蛋白质化学、免疫学和层析等很多方面的和*。http://www.thermo.com.cn/Category828.html
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DSP (Lomant's Reagent)仅用于科研
Thermo Scientific Pierce DSP (Lomant's Reagent) is an amine-reactive crosslinker having NHS-ester reactive ends and a cleavable disulfide bond in its spacer arm.
DSP contains an amine-reactive N-hydroxysuccinimide (NHS) ester at each end of an 8-carbon spacer arm. NHS esters react with primary amines at pH 7-9 to form stable amide bonds, along with release of the N-hydroxy-succinimide leaving group. Proteins, including antibodies, generally have several primary amines in the side chain of lysine (K) residues and the N-terminus of each polypeptide that are available as targets for NHS-ester crosslinking reagents. DSS, the non-cleavable analog of DSP crosslinker is also available for applications that require a stable spacer arm that cannot be cleaved in the presence of reducing agents.
DSP Highlights
- Amine-reactive NHS ester reacts rapidly with any primary amine-containing molecule
- Disulfide bond in the spacer arm is readily cleaved by 10-50 mM DTT or TCEP at pH 8.5
- Spacer arm is also cleaved with 5% β-mercaptoethanol in SDS-PAGE sample loading buffer at 100°C for 5 minutes
- Cleavable crosslinker allows separation of crosslinked products
- DSP crosslinker is membrane permeable, so intracellular crosslinking can be done
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