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PIERCE,Sulfo-SMCC, No-Weigh(tm) Format, 8 x 2 mg m

货号:22622,名称: Sulfo-SMCC, No-Weigh(tm) Format, 8 x 2 mg microtubes ,规格:8 X 2 MG
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Sulfo-SMCC, No-Weigh(tm) Format, 8 x 2 mg microtubes介绍

Thermo Scientific BCA蛋白定量产品可以用于研究蛋白:蛋白间相互作用,衡量亲和层析后的组分含量,估算从细胞裂解液里提取的膜蛋白含量和高通量筛选融合蛋白。


Sulfo-SMCC, No-Weigh(tm) Format, 8 x 2 mg microtubes现货供应



PIERCE

22622

Sulfo-SMCC, No-Weigh(tm) Format, 8 x 2 mg microtubes

8 X 2 MG

PIERCE

22980

1-ETHYL-3-(3-DIMETHYLAMINOPROPYL)CARBODIIMIDE.HCl  (EDC)

5 GM

PIERCE

22981

1-ETHYL-3-(3-DIMETHYLAMINOPROPYL)CARBODIIMIDE.HCL   (EDC)

25 GM


Sulfo-SMCC, No-Weigh(tm) Format, 8 x 2 mg microtubes品牌介绍

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Sulfo-SMCC, No-Weigh(tm) Format, 8 x 2 mg microtubes仅用于科研


Thermo Scientific Pierce Sulfo-SMCC is a very popular, water-soluble, amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a medium-length cyclohexane spacer arm (8.3 angstroms).

Sulfosuccinimidyl-4-(N-maleimidomethyl)cyclohexane-1-carboxylate (Sulfo-SMCC) is a water-soluble, non-cleavable and membrane impermeable crosslinker. It contains an amine-reactive N-hydroxysuccinimide (NHS ester) and a sulfhydryl-reactive maleimide group. NHS esters react with primary amines at pH 7-9 to form stable amide bonds. Maleimides react with sulfhydryl groups at pH 6.5-7.5 to form stable thioether bonds. The maleimide groups of Sulfo-SMCC and SMCC are unusually stable up to pH 7.5 because of the cyclohexane bridge in the spacer arm. Because it contains the hydrophilic sulfonyl moiety, Sulfo-SMCC is soluble up to ~10 mM in water and many commonly used buffers, thus avoiding the use of organic solvents which may perturb protein structure.

Sulfo-SMCC Highlights

  • Amine reactive Sulfo-NHS ester crosslinks rapidly with primary amine-containing molecule
  • Sulfhydryl-reactive maleimide reacts with cysteine residues to yield specific conjugates
  • Sulfo-SMCC is water soluble, so crosslinking can be done in physiologic solutions
  • High purity, crystalline Sulfo-SMCC can be used to create high-purity maleimide-activated derivatives
  • Cyclohexane bridge confers added stability to the maleimide group making Sulfo-SMCC the ideal crosslinking agent for maleimide activation of proteins. Maleimide groups are stable for 64 hours in 0.1 M sodium phosphate buffer, pH 7 at 4°C.


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