PIERCE,N-ETHYLMALEIMIDE
- 型号： 23030
- 更新时间： 2024-03-22
- 上一个：23011PIERCE,（MMTS） Methyl methanethiolsulfonate, 200 mg
- 下一个：23208PIERCE,Pre-Diluted Protein Assay Standards:Bovin
订货：，，
N-ETHYLMALEIMIDE介绍
Thermo Scientific BCA蛋白定量产品可以用于研究蛋白：蛋白间相互作用，衡量亲和层析后的组分含量，估算从细胞裂解液里提取的膜蛋白含量和高通量筛选融合蛋白。
N-ETHYLMALEIMIDE现货供应
PIERCE | 23030 | N-ETHYLMALEIMIDE | 25 GM |
PIERCE | 23208 | Pre-Diluted Protein Assay Standards: Bovine Serum Albumin （ | KIT |
PIERCE | 23209 | ALBUMIN STANDARD, 10 X 1 ML | 10 X 1 ML |
N-ETHYLMALEIMIDE品牌介绍
美国Pierce公司（www.pierce.com）是蛋白质化学、免疫学和层析等很多方面的和*。http://www.thermo.com.cn/Category828.html
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N-ETHYLMALEIMIDE仅用于科研
Thermo Scientific Pierce N-Ethylmaleimide （NEM） is a small compound that forms stable, covalent thioether bonds with sulfhydryls （e.g., reduced cysteines）, enabling them to be permanently blocked to prevent disulfide bond formation.
N-Ethylmaleimide （NEM） is an alkylating reagent that reacts with sulfhydryls. At pH 6.5-7.5, the maleimide reaction is specific for sulfhdryls; however, at pH values greater than 7.5, reactivity with amino groups occurs. Maleimide groups react with sulfhydryls by nucleophilic attack of the thiolate anion on one of the carbons of the double bond. When sufficient sulfhydryls have been blocked, the reaction can be monitored by measuring the decrease in absorbance at 300nm as the double bond becomes a single bond. The resulting thioether group is non-reversible and terminates in an ethyl group, blocking or capping the sulfhydryl.
Highlights:
- Permanently block sulfhydryls to prevent disulfide bond formation.
- Monitor the reaction by measuring the decrease in absorbance at 300nm
- Block sulfhydryl-containing reagents that interfere in enzyme assays
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