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PIERCE,Pierce Silver Stain for Mass Spectrometry

货号:24600,名称: Pierce Silver Stain for Mass Spectrometry ,品牌:PIERCE,规格:KIT
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Pierce Silver Stain for Mass  Spectrometry介绍

Thermo Scientific BCA蛋白定量产品可以用于研究蛋白:蛋白间相互作用,衡量亲和层析后的组分含量,估算从细胞裂解液里提取的膜蛋白含量和高通量筛选融合蛋白。

 

Pierce Silver Stain for Mass  Spectrometry现货供应

 

PIERCE

24600

Pierce Silver Stain for Mass  Spectrometry

KIT

PIERCE

24612

Pierce Silver Stain Kit

KIT

PIERCE

25202

12% Precise (tm) Protein Gels (10-well)

KIT

 

Pierce Silver Stain for Mass  Spectrometry品牌介绍

美国Pierce公司(www.pierce.com)是蛋白质化学、免疫学和层析等很多方面的先驱和*。http://www.thermo.com.cn/Category828.html

 

主要产品包括:

1)化学发光体和比色底物

 

2)分析试剂

 

3)荧光标记探针

 

4)酶联免疫分析试剂盒等

 

Pierce Silver Stain for Mass  Spectrometry仅用于科研

 

The Thermo Scientific Pierce Silver Stain for Mass Spectrometry is a complete kit for rapid and ultra-sensitive silver staining of proteins in polyacrylamide gels and efficient destaining of excised gel pieces for mass spectrometry analysis.

This silver staining kit for mass spectrometry (MS) bundles components of the high-performance Pierce Silver Stain Kit with optimized reagents to destain spots for subsequent in-gel tryptic digestion to recover peptide fragments for proteomics analysis. The resulting MS-compatible product and protocol deliver outstanding sensitivity and maintains favorable conditions for the high-yield recovery and identification (sequence coverage) of proteins by mass spectrometry. Silver staining of 2D gels is now an important intermediate step in a set of procedures that leads ultimay to identification of specific proteins in the proteome by mass fingerprinting methods. The Pierce Silver Stain protocol provides peak staining performance, flexibility, reliability and robustness for applications such as MALDI-MS.

Highlights:

  • Sensitive and fast staining – the low-background, easy-to-use silver stain provides subnanogram sensitivity, detecting proteins at less than 0.25ng per spot in 30 minutes after fixing
  • Flexible staining protocol – fix gel for 15 to 30 minutes or overnight without any affect on staining performance; stain for 1 to 30 minutes (typically 2 to 3 minutes)
  • Robust - effective silver stain for even difficult-to-stain basic proteins, such as lysozyme (pI 10) and chymotrypsinogen A (pI 9.2), which are detectable at 0.2ng and 0.5ng, respectively
  • Simple, trouble-free spot preparation – stained spots in excised gel-pieces are easily destained and made ready for tryptic digestion in one hour
  • MS compatible – reagents and protocol are optimized to provide excellent performance in mass spectrometry following extraction of peptides from stained 1D or 2D gels (SDS-PAGE)
  • Complete kit – contains all reagents necessary for staining and destaining before in-gel proteolysis and peptide recovery for analysis by mass spectrometry
  • Convenient – kit-components are stable at room temperature; saves valuable refrigerator space and eliminates the need to equilibrate reagents before use



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