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Pierce,EZ-Link (tm) Plus Activated Peroxidase Kit

货号:31489,名称: EZ-Link (tm) Plus Activated Peroxidase Kit ,品牌:PIERCE,规格:KIT
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EZ-Link (tm) Plus Activated Peroxidase Kit 介绍

Thermo Scientific BCA蛋白定量产品可以用于研究蛋白:蛋白间相互作用,衡量亲和层析后的组分含量,估算从细胞裂解液里提取的膜蛋白含量和高通量筛选融合蛋白。

 

EZ-Link (tm) Plus Activated Peroxidase Kit 现货供应

 

PIERCE

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EZ-Link (tm) Maleimide Activated Horseradish Peroxidase Kit

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EZ-Link (tm) Activated Peroxidase Antibody Labeling Kit

1 KIT

 

EZ-Link (tm) Plus Activated Peroxidase Kit 品牌介绍

美国Pierce公司(www.pierce.com)是蛋白质化学、免疫学和层析等很多方面的先驱和*。http://www.thermo.com.cn/Category828.html

 

主要产品包括:

1)化学发光体和比色底物

 

2)分析试剂

 

3)荧光标记探针

 

4)酶联免疫分析试剂盒等

 

EZ-Link (tm) Plus Activated Peroxidase Kit 仅用于科研

 

Pierce Plus Activated Peroxidase for making antibody-HRP conjugates.

Thermo Scientific Pierce Plus Activated Peroxidase is an amine-reactive form of horseradish peroxidase (HRP) that provides coupling efficiencies of greater than 95% with antibodies and other proteins!

Sugar groups on native HRP have been oxidized with periodate to produce amine-reactive aldehydes. The activated peroxidase spontaneously and efficiently crosslink with primary amines on antibody or other proteins. The method is more effective than other amine-reactive chemistries, such as glutaraldehyde coupling, which often causes polymerization and conjugate inactivation.

This high-quality prepared enzyme eliminates the difficulties inherent in preparing and validating activated peroxidase from scratch. The kit provides the enzyme, accessory reagents and protocol to easily produce high-performance conjugates for Western blotting, ELISA and other detection techniques.

Highlights:

  • Reacts with readily accessible primary amines to form covalent secondary amine bonds with antibodies or other proteins
  • Provides more efficient and stable crosslinking than glutaraldehyde methods, resulting in conjugates having higher activity and overall performance in most applications
  • Can be stored for at least 12 months at -20°C
  • Enzyme activity is 120 to 200 units/mg



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