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Pierce,AminoLink Plus Immobilization Kit

货号:44894,名称: AminoLink Plus Immobilization Kit ,品牌:PIERCE,规格:1 KIT
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AminoLink Plus Immobilization Kit 介绍

Thermo Scientific BCA蛋白定量产品可以用于研究蛋白:蛋白间相互作用,衡量亲和层析后的组分含量,估算从细胞裂解液里提取的膜蛋白含量和高通量筛选融合蛋白。

 

AminoLink Plus Immobilization Kit 现货供应

 

PIERCE

44894

AminoLink Plus Immobilization Kit

1 KIT

PIERCE

44897

IgM Purification Kit

EA

PIERCE

44985

Pierce Fab Preparation Kit,   10 rnxs, (0.25-4mg Ab per rxn)

KIT

 

AminoLink Plus Immobilization Kit 品牌介绍

美国Pierce公司(www.pierce.com)是蛋白质化学、免疫学和层析等很多方面的先驱和*。http://www.thermo.com.cn/Category828.html

 

主要产品包括:

1)化学发光体和比色底物

 

2)分析试剂

 

3)荧光标记探针

 

4)酶联免疫分析试剂盒等

 

AminoLink Plus Immobilization Kit 仅用于科研

 

AminoLink Plus Coupling Resin and Immobilization Kits

Thermo Scientific AminoLink Plus Coupling Resin and Immobilization Kits use activated beaded agarose and a robust coupling chemistry to immobilize proteins and other ligands through primary amines (–NH2) to the resin.

Once an antibody or other ligand is immobilized, the prepared affinity resin can be used for a variety of affinity purification methods involving batch or column chromatography. The resin and linkage are stable in binding and elution conditions typically used in affinity chromatography, enabling prepared resin to be used for at least 10 rounds of affinity purification.

Highlights:

  • AminoLink Plus Coupling Resin – aldehyde-activated crosslinked 4% beaded agarose
  • Ideal for antibodies and other proteins – immobilize molecules via primary amines (-NH2)
  • Flexible coupling conditions – efficient (>85%) coupling over a wide range of pH (4-10) and buffer conditions (PBS or other non-amine buffer with or without organic solvent); regular (PBS, pH 7.2) and enhanced (citrate-carbonate, pH 10) coupling protocols provided
  • Stable, permanent immobilization – Coupling reaction esults in stable, leak-resistant secondary amine bond between resin and ligand
  • Better than immobilization to CNBr-activated agarose – bond is more stable and uncharged, resulting in less nonspecific binding in affinity purification procedures
  • Versatile and reusable – prepared affinity resin is adaptable to column and batch affinity techniques and the resin is reusable for typical applications based on protein binding interactions
  • Convenient kits and product sizes –choose 1- or 5-column kit containing complete sets of buffers, reagents and versatile spin/drip columns, or select bulk resin.



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