重组人转化生长因子b3 (rHuTGFb3)
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- 更新时间: 2025-02-12
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产品介绍
CYT-368 | 重组人转化生长因子b3 (rHuTGFb3) | 1ug/5ug | Prospec |
Background: | |
Transforming growth factor-betas (TGF betas) are multifunctional peptides that regulate growth and differentiation in a variety of cells. The transforming growth factor beta (TGF-beta) family of growth modulators play critical roles in tissue development and maintenance. Recent data suggest that individual TGF-beta isoforms (TGF-beta 1, -beta 2 and -beta 3) have overlapping yet distinct biological actions and target cell specificities, both in developing and adult tissues Transforming growth factor (TGF) beta 3 is a new isoform of the TGF beta superfamily and is presumed to play an important role in wound repair and scarring. TGF-beta 3 is believed to be involved in osteoblast proliferation, chemotaxis, and collagen synthesis. | |
![]() | Description : |
Recombinant Human TGF-b 3 produced in E.Coli is a disulfide-linked homodimeric, non-glycosylated, polypeptide chain containing 112 amino acids and having a molecular mass of 25,445 Dalton. Recombinant Human TGF-b 3 is purified by standard chromatographic techniques. | |
![]() | Physical Appearance: |
Sterile Filtered clear solution. | |
![]() | Formulation: |
The protein solution (0.25mg/ml) contains 20% Ethanol and 0.12% Acetic acid. | |
![]() | Stability: |
Recombinant Human TGF-beta 3 although stable at room temperature for 3 weeks, should be stored at 40C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). | |
![]() | Purity: |
Greater than 99.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel. | |
![]() | Amino acid sequence: |
The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Leu-Asp-Thr-Asn. | |
![]() | Aggregates: |
Less than 1% as determined by silver-stained SDS-PAGE gel analysis. | |
![]() | Biological Activity: |
ProSpec’s TGF-beta 3 is fully biologically active when compared to standard. The ED50 as determined by the cell toxicity assay using the WHO Standard 98/608 as a direct comparison is < 0.05ng/ml, corresponding to a Specific Activity of 12.5 x 106IU/mg. | |
![]() | Endotoxin: |
Less than 0.1 ng/µg (IEU/µg) of Recombinant Human TGF-beta 3. | |
![]() | Protein content: |
Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 1.718 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (InliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of TGF-beta 3 as a Reference Standard. | |
![]() | Usage: |
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
佰易聚生物商城提供美国光谱医学透析袋,wako生化试剂,amresco生化试剂,sigma生化试剂,优质的分子生物学试剂和试剂盒、培养基本和培养耗材、细胞因子和细胞分离液、抗体和elisa试剂盒及常用生物试剂等,大量现货火爆*,欢迎登录 www.ebioeasy.com或者,,洽谈选购!
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