重组人细胞毒性T淋巴细胞相关抗原-4rHuCTLA-4）
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- 更新时间： 2025-02-12
- 上一个：重组人前骨形态发生蛋白-2（rHuProBMP-2）
- 下一个：重组人可溶性CD4 （rsCD4）
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产品介绍
| CYT-366 | 重组人细胞毒性T淋巴细胞相关抗原-4rHuCTLA-4） | 20μg/100μg/1mg | Prospec |
| Background: | |
| CTLA-4 is expressed in low copy number by T-cells only after activation, but it binds CD28-ligand with approximay 20-fold higher affinity than CD28. A soluble form of the extracellular domain of CTLA-4 has been shown to bind CD28-ligand with high avidity and to suppress T-cell-dependent antibody responses in vivo. Large doses of this soluble protein also suppress responses to a second immunization. | |
 | Description : |
| Recombinant Human CTLA-4 produced in insect cells is a homodimeric, glycosylated, polypeptide chain containing a total molecular mass of 80 kDa. Each subunit （40kDa） is fused to a polypeptide linker to the Fc portion of Human IgG1. CTLA-4 is expressed in low copy number by T-cells only after activation, but it binds CD28-ligand with approximay 20-fold higher affinity than CD28. A soluble form of the extracellular domain of CTLA-4 has been shown to bind CD28-ligand with high avidity and to suppress T-cell-dependent antibody responses in vivo. Large doses of this soluble protein also suppress responses to a second immunization. Recombinant Human CTLA-4 is purified by proprietary chromatographic techniques. | |
| Physical Appearance: |
| Sterile Filtered White lyophilized （freeze-dried） powder. | |
| Formulation: |
| Recombinant CTLA-4 was lyophilized with no additives. | |
| Solubility: |
| It is recommended to reconstitute the lyophilized CTLA4 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. | |
| Stability: |
| Lyophilized CTLA4 although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution CTLA-4 should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Please avoid freeze-thaw cycles. | |
| Purity: |
| Greater than 95.0% as determined by: （a） Analysis by RP-HPLC. （b） Anion-exchange FPLC. （c） Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel. | |
| Amino acid sequence: |
| The sequence of the amino acids was determined and was found to be: Lys-Ala-Met-His-Val. | |
| Dimers and aggregates: |
| Less than 1% as determined by silver-stained SDS-PAGE gel analysis. | |
| Biological Activity: |
| ProSpec’s Recombinant CTLA-4 is fully biologically active when compared to standards. | |
| Endotoxin: |
| Less than 0.1 ng/µg （IEU/µg） of CTLA-4. | |
| Protein content: |
| Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280. 2. Analysis by RP-HPLC, using a standard solution of Human CTLA-4 as a Reference Standard. | |
| Usage: |
| Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
佰易聚生物商城提供美国光谱医学透析袋，wako生化试剂，amresco生化试剂，sigma生化试剂，优质的分子生物学试剂和试剂盒、培养基本和培养耗材、细胞因子和细胞分离液、抗体和elisa试剂盒及常用生物试剂等,大量现货火爆*,欢迎登录 www.ebioeasy.com或者,，洽谈选购！
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