重组人粒-巨噬细胞集落刺激因子-sf9（rHuGM-CSF-Sf9）
- 型号：
- 更新时间： 2025-02-12
- 上一个：重组人粒细胞巨噬细胞集落刺激因子 （GM-CSF）
- 下一个：重组猪粒-巨噬细胞集落刺激因子（rpGM-CSF）
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产品介绍
| CYT-416 | 重组人粒-巨噬细胞集落刺激因子-sf9（rHuGM-CSF-Sf9） | 2μg/10μg/1mg | Prospec |
| Description : | |
| Recombinant Human GM-CSF produced in insect cells is a single, glycosylated, polypeptide chain containing 127 amino acids and having a molecular mass of 14477 Dalton. Recombinant Human GM-CSF is fused to a C-terminal His -tag （6x His） and purified by proprietary chromatographic techniques. | |
 | Physical Appearance: |
| Sterile Filtered White lyophilized （freeze-dried） powder. | |
| Formulation: |
| The protein was lyophilized with no additives. | |
| Solubility: |
| It is recommended to reconstitute the lyophilized GM-CSF in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. | |
| Stability: |
| Lyophilized GM-CSF although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution GM-CSF should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Please avoid freeze-thaw cycles. | |
| Purity: |
| Greater than 98.0% as determined by: （a） Analysis by RP-HPLC. （b） Anion-exchange FPLC. （c） Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel. | |
| Amino acid sequence: |
| The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Pro-Ala-Arg-Ser. N-terminal methionine has been compley removed enzymatically. | |
| Dimers and aggregates: |
| Less than 1% as determined by silver-stained SDS-PAGE gel analysis. | |
| Biological Activity: |
| ProSpec’s Recombinant GM-CSF is fully biologically active when compared to standard. The ED50 as determined by the dose-dependant stimulation of the proliferation of human TF-1 cells （human erythroleukemic indicator cell line） is < 0.1 ng/ml, corresponding to a Specific Activity of 1 x107 IU/mg. | |
| Endotoxin: |
| Less than 0.1 ng/µg （IEU/µg） of GM-CSF . | |
| Protein content: |
| Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 0.963 as the extinction coefficient for a 0.1% （1mg/ml） solution. This value is calculated by the PC GENE computer analysis program of protein sequences （InliGenetics）. 2. Analysis by RP-HPLC, using a standard solution of GM-CSF as a Reference Standard. | |
| Usage: |
| Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
佰易聚生物商城提供美国光谱医学透析袋，wako生化试剂，amresco生化试剂，sigma生化试剂，优质的分子生物学试剂和试剂盒、培养基本和培养耗材、细胞因子和细胞分离液、抗体和elisa试剂盒及常用生物试剂等,大量现货火爆*,欢迎登录 www.ebioeasy.com或者,，洽谈选购！
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