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EZ-Link(R) NHS-PEG4-Biotin,No-Weigh(tm) Format,

货号:21329,名称:EZ-Link(R) NHS-PEG4-Biotin,No-Weigh(tm) Format,品牌:PIERCE,规格:8 X 2 MG
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产品介绍

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EZ-Link(R) NHS-PEG4-Biotin,No-Weigh(tm) Format介绍

 

Thermo Scientific BCA蛋白定量产品可以用于研究蛋白:蛋白间相互作用,衡量亲和层析后的组分含量,估算从细胞裂解液里提取的膜蛋白含量和高通量筛选融合蛋白。

 

 

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EZ-Link(R) NHS-PEG4-Biotin,No-Weigh(tm) Format现货供应

 

PIERCE

21329

EZ-Link(R) NHS-PEG4-Biotin,No-Weigh(tm) Format,

8 X 2 MG

PIERCE

21331

EZ-Link (tm) Sulfo-NHS-SS-Biotin, 100 mg

100 MG

PIERCE

21335

EZ-Link (tm) Sulfo-NHS-LC-Biotin, 100 mg

100 MG

 

EZ-Link(R) NHS-PEG4-Biotin,No-Weigh(tm) Format品牌介绍

 

美国Pierce公司(www.pierce.com)是蛋白质化学、免疫学和层析等很多方面的先驱和*。http://www.thermo.com.cn/Category828.html

 

主要产品包括:

1)化学发光体和比色底物

 

2)分析试剂

 

3)荧光标记探针

 

4)酶联免疫分析试剂盒等

 

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EZ-Link(R) NHS-PEG4-Biotin,No-Weigh(tm) Format

仅用于科研

 

Thermo Scientific EZ-Link NHS-PEG4-Biotin is a pegylated, water-soluble reagent for simple and efficient biotin labeling of antibodies, proteins and other primary amine-containing macromolecules.

The N-hydroxysuccinimide ester (NHS) group reacts specifically and efficiently with lysine and N-terminal amino groups at pH 7-9 to form stable amide bonds. The hydrophilic polyethylene glycol (PEG) spacer arm of this biotinylation reagent imparts water solubility that is transferred to the biotinylated molecule, thus reducing aggregation of labeled proteins stored in solution (read more about PEGylation). The PEG spacer arm also gives the reagent a long and flexible connection to minimize steric hindrance involved with binding to avidin molecules.

Highlights:

  • Label almost any protein with biotin to facilitate immobilization, purification or detection
  • Amine-reactive NHS ester reacts rapidly with any primary amine-containing molecule to attach the biotin label via a stable amide bond
  • Reagent is water soluble and membrane impermeable – suitable for cell surface labeling
  • PEGylation increases solubility and helps prevent aggregation of labeled molecules
  • Long spacer arm reduces steric hindrance when binding to avidin molecules
  • No-Weigh Format saves time, preserves reagent activity and prevents cross-contamination between uses
  • Reactive group: N-hydroxysuccinimide ester (NHS ester)
  • Reactive toward: primary amines, as in side chain of lysine (K) and N-terminus of polypeptides



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