你的位置:首页 >产品展示 > 生化试剂 > 琼脂糖 > 21650PIERCE,Sulfo-LC-SPDP, sulfosuccinimidyl 6-(3'-(2 p

PIERCE,Sulfo-LC-SPDP, sulfosuccinimidyl 6-(3'-(2 p

货号:21650,名称:Sulfo-LC-SPDP, sulfosuccinimidyl 6-(3'-(2 pyridyldithio) pro,品牌:PIERCE,规格:50 MG
  • 在线订购
产品介绍

订货:

 

Sulfo-LC-SPDP, sulfosuccinimidyl 6-(3'-(2 pyridyldithio) pro介绍

 

Thermo Scientific BCA蛋白定量产品可以用于研究蛋白:蛋白间相互作用,衡量亲和层析后的组分含量,估算从细胞裂解液里提取的膜蛋白含量和高通量筛选融合蛋白。

Sulfo-LC-SPDP, sulfosuccinimidyl 6-(3'-(2 pyridyldithio) pro现货供应

 

PIERCE

21627

Streptavidin, R-Phycoerythrin Conjugated, 1 mL.

1 ML

PIERCE

21650

Sulfo-LC-SPDP, sulfosuccinimidyl 6-(3'-(2 pyridyldithio) pro

50 MG

PIERCE

21655

DISUCCINIMIDYL SUBERATE, (DSS)50 MG

50 MG

 

Sulfo-LC-SPDP, sulfosuccinimidyl 6-(3'-(2 pyridyldithio) pro品牌介绍

 

美国Pierce公司(www.pierce.com)是蛋白质化学、免疫学和层析等很多方面的先驱和*。http://www.thermo.com.cn/Category828.html

 

主要产品包括:

1)化学发光体和比色底物

 

2)分析试剂

 

3)荧光标记探针

 

4)酶联免疫分析试剂盒等

 

Sulfo-LC-SPDP, sulfosuccinimidyl 6-(3'-(2 pyridyldithio) pro

仅用于科研

 

Thermo Scientific Pierce Sulfo-LC-SPDP is a water-soluble, long-chain crosslinker for amine-to-sulfhydryl conjugation via NHS-ester and pyridyldithiol reactive groups that form cleavable (reducible) disulfide bonds with cysteines.

 

Sulfo-LC-SPDP is Sulfosuccinimidyl 6-(3'-[2-pyridyldithio]-propionamido)hexanoate, a heterobifunctional, thiol-cleavable and membrane permeable crosslinker. It contains an amine-reactive N-hydroxysuccinimide (NHS) ester that will react with lysine residues to form a stable amide bond. The other end of the spacer arm is terminated in the pyridyl disulfide group that will react with sulfhydryls to form a reversible disulfide bond.

 

Highlights:

  • Releases a detectable by-product when reacted with a free sulfhydryl group; by measuring the release of pyridine-2-thione at 343 nm, the reaction can be easily followed
  • Water soluble, amine reactive Sulfo-NHS ester reacts rapidly with any primary amine-containing molecule
  • Disulfide bond in the spacer arm is readily cleaved by 10-50 mM DTT or TCEP at pH 8.5
  • Spacer arm is also cleaved with 5% β-mercaptoethanol in SDS-PAGE sample loading buffer at 100°C for 5 minutes
  • Cleavable crosslinker allows separation of crosslinked products

     

     

  • Sulfo-LC-SPDP crosslinker is not membrane permeable, so crosslinking can be carried out on the cell surface

     

     



更多产品
在线订购

留言框

  • 产品:

  • 留言内容:

  • 您的单位:

  • 您的姓名:

  • 联系电话:

  • 常用邮箱:

  • 详细地址:

  • 省份:

  • 验证码:

    请输入计算结果(填写阿拉伯数字),如:三加四=7