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Guardian (tm) Peroxidase Conjugate Stabilizer Diluent, 1 L 介绍

Thermo Scientific BCA蛋白定量产品可以用于研究蛋白：蛋白间相互作用，衡量亲和层析后的组分含量，估算从细胞裂解液里提取的膜蛋白含量和高通量筛选融合蛋白。

Guardian (tm) Peroxidase Conjugate Stabilizer Diluent, 1 L 现货供应

Guardian (tm) Peroxidase Conjugate Stabilizer Diluent, 1 L 品牌介绍

美国Pierce公司(www.pierce.com)是蛋白质化学、免疫学和层析等很多方面的和*。http://www.thermo.com.cn/Category828.html

主要产品包括：

1)化学发光体和比色底物

2)分析试剂

3)荧光标记探针

4)酶联免疫分析试剂盒等

Guardian (tm) Peroxidase Conjugate Stabilizer Diluent, 1 L 仅用于科研

There's no need to worry about your antibody or streptavidin peroxidase conjugates becoming inactive over time when you use new Guardian Peroxidase Stabilizer/Diluent (S/D), which preserves peroxidase activity from 1:100 to 1:100,000 dilution (Figures 1 and 3). Guardian S/D Formulation can be used to create stock solutions (1:1,000) or ready-to-use assay reagents (1:100,000) that can be stored at 4°C for 12 months or at room temperature for six months. No more wasting money with HRP dilutions you throw away the same day.

This Guardian Formulation is ideal as an assay diluent in ELISA and solution-based assays. If you are using a membrane-based assay, Pierce recommends diluting the Guardian-stabilized HRP conjugate further in assay buffer. Figure 2 demonstrates the activity of a streptavidin-HRP conjugate stored in Guardian Stabilizer/Diluent at a concentration of 10µg/mL. This HRP conjugate is further diluted (1:300) to perform the Northern blot assay. The Streptavidin-HRP is still able to detect a biotinylated RNA probe even after 12 months of storage at 4°C at a highly dilute concentration.

Highlights:

• Preserves HRP activity – no significant loss of HRP activity over a six-month period at room temperature (1:1,000 dilution) or 12 months at 4°C
• Convenient – store ready-to-use dilutions (1:1,000-1:100,000) that maintain enzyme activity in the refrigerator – no aliquoting or freezing
• Assay compatible – simply add your favorite blocking buffer to create the ideal diluent for your HRP-based ELISA system or store the HRP conjugate as a 1:1,000 stock solution for Western blots and dilute in the final assay buffer.
• Saves money – less expensive than competitor formulations or ordering new HRP conjugates